Primer Annealing Temperature Calculator How To Calculate Primer Annealing Temperature?

How to calculate primer annealing temperature? - primer annealing temperature calculator

As the primer annealing temperature to calculate? Please go ahead and the appropriate form (manually)? In my experience with the DNA template and primer base pairs 18 to 25 base pairs (minimum size = 18 first and height of the first maximum is 25). thank you reply!